The effects of organo-mineral nutritional regimes on malus domestica productivity and microbiological markers in southeast Kazakhstan

This study evaluates the effects of organo-mineral fertilization systems combined with biological preparations on the productivity and soil microbiological indicators of Golden Delicious apple trees in southeastern Kazakhstan. Using a randomized block design, six fertili-zation variants— including organic, bioorganic, and mineral treatments - were applied to irrigated light brown soils with low organic matter content. Results demonstrated that treatments with biohumus and BioSok Energy Plus significantly increased yields (39–64%,) sugar content (0.5–1.9%), dry matter (0.8–1.5%), and fruit shelf life compared to controls. Additionally, these treat-ments enhanced the abundance of beneficial soil microorganisms, indicating improved soil biological activity and structure. The findings suggest that integrating organic and bioorganic fertilizers with mineral nutrition promotes sustainable orchard productivity by boosting soil fertility and microbiocenosis, thereby supporting long-term soil health and sustainable horticultural practices in arid regions.

1. Alhassan Y.A., Haruna Y., Firdausi S.K., Muhammad M.A. Economics of bio-based fertilizer in improving crop productivity through extension services delivery // International Journal of Agriculture and Plant Science. – 2019. – Vol. 1. – P. 10–13.

2. Ye L., Zhao X., Bao E. et al. Bio-organic fertilizer with reduced rates of chemical fertilization improves soil fertility and enhances tomato yield and quality // Scientific Re-ports. – 2020. – Vol. 10. – Art. 177. – DOI: https://doi.org/10.1038/s41598-019-56954-2.

3. Baishanova A.E., Kedelbaev B.Sh. Problems of soil degradation. Analysis of the current state of fertility of irrigated soils in the Republic of Kazakhstan // Scientific Review. Biological Sciences. – 2016. – P. 5–13. – URL: https://science-biology.ru/ru/article/view?id=991 (accessed: 19.12.2025).

4. Zhandybayev O., Malimbayeva A., Shibikeyeva A., Amirkhan A. Effect of fertigation on nutrient dynamics of gray-brown soils and apple (Malus pumila) yields in intensive orchards of Kazakhstan // Research on Crops. – 2023. – Vol. 24. – P. 506–514. – DOI: 10.31830/2348-7542.2023.ROC-927.

5. Minasny B., Malone B., McBratney A. et al. Soil carbon 4 per mille // Geoderma. – 2017. – Vol. 292. – P. 9–89. – DOI: 10.1016/j.geoderma.2017.01.002.

6. Holik L., Hlisnikovský L., Honzik R., Trögl J., Burdová H., Popelka J. Soil microbial communities and enzyme activities after long-term application of inorganic and organic fertilizers at different depths of the soil profile // Sustainability. – 2019. – Vol. 11, № 12. – P. 3251.

7. Sivojiene D., Kacergius A., Baksiene E., Maseviciene A., Zickiene L. The influence of organic fertilizers on the abundance of soil microorganism communities, agrochemical indicators, and yield in East Lithuanian light soils // Selected Papers from Conference of CYSENI. – 2021. – Vol. 10, № 12. – P. 2648.

8. Zhang J., Bei Sh., Li B., Zhang J., Christie P., Li X. Organic fertilizer, but not heavy liming, enhances banana biomass, increases soil organic carbon and modifies soil microbiota // Applied Soil Ecology. – 2019. – Vol. 136. – P. 67–79. – DOI: 10.1016/j.apsoil.2018.12.017.

9. Kapłan M., Klimek K., Borkowska A., Buczynski K. Effect of growth regulators on the quality of apple tree whorls // Applied Sciences. – 2023. – Vol. 13. – Art. 11472. – DOI: 10.3390/app132011472.

10. Ponchia G., Fila G., Gardiman M. Effects of root-stock and interstem on growth, productivity and mineral nutrition of ‘Golden Delicious’ apple trees // Acta Horticulturae (ISHS). – 1997. – № 448. – P. 107–112. – URL: https://www.researchgate.net/publica-tion/286202731 (accessed: 20.12.2025).

11. Novitsky M.V., Lavrishchev A.V., Nazarova A.V. et al. Laboratory and practical classes in soil science. – St. Petersburg: Prospekt Nauki, 2024. – 320 p. – URL: https://www.ibooks.ru/bookshelf/353755/reading (accessed: 01.12.2025).

12. Methodological recommendations (2004). Methods of microbiological control of soil. – Moscow: Federal Center for State Sanitary and Epidemiological Surveillance of the Ministry of Health of the Russian Federation, 2004. – 21 p. – URL: https:// https://meganorm.ru/Data2/1/4293737/4293737876.pdf (accessed: 01.12.2025).

13. GOST 17.4.3.01-83. Environmental protection. Soils. General requirements for sampling. – Mode of access: https://allgosts.ru/13/080/gost_17.4.3.01-83 (accessed: 01.01.2026).

14. ISO 10576:2022. Statistical methods. Guidelines for the evaluation of conformity with specified requirements. – Geneva: International Organization for Standardization, 2022.

15. Khan M.F., Hof C., Niemcová P., Murphy C.D. Recent advances in fungal xenobiotic metabolism: enzymes and applications // World Journal of Microbiology and Biotechnology. – 2023. – Vol. 39, No. 11. – Art. 296. – DOI: 10.1007/s11274-023-03737-7.

16. Kumari R., Singh A., Yadav A.N. Fungal enzymes: degradation and detoxification of organic and inorganic pollutants // Yadav A.N. (ed.) Recent Trends in Mycological Research. Fungal Biology. – Cham: Springer, 2021. – Vol. 2. – P. 99–125. – DOI: 10.1007/978-3-030-68260-6_5.

17. Heidarzadeh A. Retracted article: Role of amino acids in plant growth, development, and stress responses: a comprehensive review // Discover Plants. – 2025.

18. – Vol. 2. – P. 237. – DOI: 10.1007/s44372-025-00322-0.

19. Igamberdiev A.U., Bykova N.V. Role of organic acids in the integration of cellular redox metabolism and mediation of redox signalling in photosynthetic tissues of higher plants // Free Radical Biology and Medicine. – 2018. – Vol. 122.

20. Zhang L., Zhang J., Guo J. Relationships between organic acid metabolism and the accumulation of sugars and calcium in fruits of Cerasus humilis during different deve-lopment stages // Plants. – 2024. – Vol. 13. – P. 3053. – DOI: 10.3390/plants13213053.

21. Li M., Zhu Y., Li S., Zhang W., Yin C., Lin Y. Regulation of phytohormones on the growth and development of plant root hair // Frontiers in Plant Science. – 2022. – Vol. 13. – Art. 865302. – DOI: 10.3389/fpls.2022.865302.

22. Fitzpatrick T.B. B vitamins: an update on their importance for plant homeo-stasis // Annual Review of Plant Biology. – 2024. – Vol. 75. – P. 67–93. – DOI: 10.1146/annurev-arplant-060223-025336.

23. Tariq L., Bi Y., Wang H., Guo M., Song F. Balancing vitamin B6 homeostasis for plant immunity // Physiologia Plantarum. – 2025. – Vol. 177, № 4. – Art. e70415. – DOI: 10.1111/ppl.70415.

24. Etienne P., Diquelou S., Prudent M., Salon C., Maillard A., Ourry A. Macro and micronutrient storage in plants and their remobilization when facing scarcity: the case of drought // Agriculture. – 2018. – Vol. 8. – Art. 14. – DOI: 10.3390/agriculture8010014.

25. Kumar S., Kumar S., Mohapatra T. Interaction between macro- and micro-nutrients in plants // Frontiers in Plant Science. – 2021. – Vol. 12. – Art. 665583. – DOI: 10.3389/fpls.2021.665583.

26. Mosaad I.S.M., Selim E.M.M., Gaafar D.E.M. et al. Effects of humic and fulvic acids on forage production and grain quality of triticale under various soil salinity levels // Cereal Research Communications. – 2025. – Vol. 53. – P. 1811–1829. – DOI: 10.1007/s42976-024-00609-0.

27. Nabi F., Sarfaraz A., Kama R., Kanwal R., Li H. Structure-based function of humic acid in abiotic stress alleviation in plants: a review // Plants (Basel). – 2025. – Vol. 14, № 13. – Art. 1916. – DOI: 10.3390/plants14131916.

28. Gu S., Hu Q., Cheng Y. et al. Application of organic fertilizer improves microbial community diversity and alters microbial network structure in tea (Camellia sinensis) plantation soils // Soil and Tillage Research. – 2019. – Vol. 195. – Art. 104356. – DOI: 10.1016/j.still.2019.104356.

29. Ansabayeva A., Makhambetov M., Rebouh N.Y. et al. Plant growth-promoting microbes for resilient farming systems: mitigating environmental stressors and boosting crops productivity – a review // Horticulturae. – 2025. – Vol. 11. – Art. 260. – DOI: 10.3390/horticulturae11030260.

30. Santoyo G., Guzmán-Guzmán P., Parra-Cota F.I. et al. Plant growth stimulation by microbial consortia // Agronomy. – 2021. – Vol. 11. – P. 219. – DOI: 10.3390/agronomy11020219.

31. Assefa S., Tadesse S. The principal role of organic fertilizer on soil properties and agricultural productivity – a review // Agricultural Research & Technology: Open Access Journal. – 2019. – Vol. 22. – Art. 556192.

32. Bamdad H., Lazarovits G., Franco B.B. Soil amendments for sustainable agriculture: microbial organic fertilizers // Soil Use and Management. – 2022. – Vol. 38, No. 1. – P. 94–120. – DOI: 10.1111/sum.12762.

33. Ivanov A.L., Kogut B.M., Semenov V.M., Turina Oberlander M., Waksman Schanbacher N. The development of theory on humus and soil organic matter: from Turin and Waksman to present days // Dokuchaev Soil Bulletin. – 2017. – № 90. – P. 3–38.

34. – DOI: 10.19047/0136-1694-2017-90-3-38.