Influence of high substrate concentrations on catalase reaction in loamy soil (Ygdir province, Turkey). II. Determination of the kinetic parameters of the enzyme catalases in soil

   In this study, analyzes were performed at various concentrations (3, 6, 9, 12, 15, 18, 21, 24, 30 %) of the H2O2 substrate to calculate the kinetic parameters of the soil catalase enzyme (Vmax, KM, Vmax / KM, KSEE, [S]opt and υ0,max). In order to calculate these parameters, the velocity values (v) were first calculated using the results of the analysis. Various models were then defined expressing the product-substrate relationship: [P] = f([S]). Further, according to the model selection criteria (R2, R2adj, σ, A, D, UII, AIC), the most suitable model was determined, reflecting the ratio [P] = f([S]). The initial speed in accordance with the established model was calculated by the formula: υ0=d([P])/dt│t=0. As a result of the study, it was found that salinity and alkalinity reduce the activity of catalase. As the substrate concentration increased, significant inhibition of the catalase enzymatic reaction was observed. The values of the coefficient of decomposition (KESS) of the enzymatic reaction are higher in saline soils, that is, inhibition of the formation of products is observed.

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