EFFECT OF INOCULATES ON ABUNDANCE OF ACTINOMYCETES IN ALFALFA RHISOSPHERE

This paper presents the results of the impact of microbial inoculants on the number of actinomycetes in the rhizosphere soil of alfalfa (Medicago sativa L.). This plant species in addition to high yield potential and quality of biomass, is characterized by an intense process of nitrogen fixation. The rhizosphere of alfalfa abounds in numerous microorganisms. The aim of the research was to investigate the effect of inoculating with two nitrogen-fixing bacteria (Sinorhizobium meliloti and Azotobacter chroococcum) and two isolates (CC657 and Coll11) ofthe phytopathogen fungus Colletotrichum destructivum on the number actinomycetes in the rhizospheric soil of alfalfa varieties (Affinity, Perry and K-28). The highest number of actinomycetes was determined in rhisosphere of cultivar Affinity which was inoculated with A. chroococcum + isolate Coll-11, while the lowest number was found in rhisosphere of cultivar K-28 inoculate with CC657. Results of Fisher test shows the absence of statistically significant differences in abundance of actinomycetes between treatments, meaning there were four homogenous groups: 1. treatments of cultivar K-28 with Coll-11 and CC657 + S. meliloti; 2. treatments of cultivar K-28 with Coll 11 + S. meliloti and control treatment (without inoculation) in cultivar Perry; 3. treatments with Coll 11 + A. chroococcum in cultivar K-28 and CC657 + A. chroococcum in cultivar Perry; 4. inoculation with CC657 + A. chroococcum in K-28 and Coll 11 + A. chroococcum in cultivar Perry.

Rhizosphere, fungi, actinomycetes, alfalfa

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