kaz65Почвоведение и агрохимияSoil Science and Agrichemistry1999-740X2959-3433Казахский научно-исследовательский институт почвоведения и агрохимии имени У.У. Успанова10.51886/1999-740X_2023_1_53kaz65-717Research ArticleБиология почвSoil biologyБиоремедиация почв, загрязненных катионами никеля с использованием бактерийBioremediation of Ni (II) cation-contaminated soils by bacteriaУсмонкуловаА.UsmonkulovaA.

100128, г Ташкент, ул. А. Кадыри, 7Б

Usmonkulova Aziza - PhD student, Junior researcher at the Phytovirology laboratory

100128, Tashkent, A. Kadyri str. 7B

usmonkulova.aziza@mail.ruИнститут микробиологии Академии наук УзбекистанаУзбекистанInstitute of Microbiology of the Academy of Sciences of UzbekistanUzbekistan202315042023015362Copyright © Усмонкулова А., 20232023Усмонкулова А.Usmonkulova A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.soil.kz/jour/article/view/717

Взаимодеиствие бактерий, стимулирующих рост растений, с растениями и их способность очищать загрязненную почву в последние годы привлекают все больше внимания. В этом исследовании были изучены три штамма ризобактерий (Enterobacter ludwigii, Enterobacter cloacae и Pseudomonas aeruginosa) для определения их индивидуального и комбинированного синергетического воздействия на ремедиацию почв, загрязненных никелем. В качестве опытного растения использовалась пшеница. Проростки пшеницы высевали в почву, содержащую 57,42; 95,7 и 191,4 мг/кг никеля, а затем подвергали 30-дневной обработке смесью ризосферных бактерий Enterobacter ludwigi, Enterobacter cloacae и Pseudomonas aeruginosa. Затем измеряли высоту растений, биомассу в стеблях и корнях и содержание хлорофилла. Было обнаружено, что характеристики роста растений значительно улучшались, когда использовался консорциум из трех бактериальных штаммов, в отличие от результата, когда использовался только один штамм. Результаты продемонстрировали потенциал синергетической биоремедиации почв, загрязненных никелем, и улучшения развития растений среди изученных ризобактерий. Результаты этого исследования предлагают убедительные доказательства использования недорогой, высокоэффективной биоремедиации на основе микроорганизмов для почв, загрязненных никелем.

The interaction of plant growth-promoting bacteria with plants and their ability to clean up contaminated soil has attracted more attention in recent years. In this study, three rhizobacteria strains (Enterobacter ludwigi, Enterobacter cloacae, and Pseudomonas aeruginosa) were examined to determine their individual and combined synergistic effects on the remediation of Ni -contaminated soils. Wheat was used as a test plant. Wheat seedlings were sown in soils containing 57.42, 95.7, and 191.4 mg/kg of nickel and were then given a 30-day treatment with a mixture of the rhizosphere bacteria Enterobacter ludwigi, Enterobacter cloacae, and Pseudomonas aeruginosa. Next, plant height, biomass in the stems and roots, and chlorophyll content were measured. It was discovered that plant growth characteristics were greatly improved when a consortium of three bacterial strains was used as opposed to the outcome when only one strain was used. The outcomes demonstrated the potential for synergistic bioremediation of Ni-contaminated soils and enhancement of plant development among the studied rhizobacteria. The findings of this study offer solid evidence in using an inexpensive, highly efficient microorganism-based bioremediation for soils contaminated with nickel.

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The authors declare that there are no conflicts of interest present.