kaz65Почвоведение и агрохимияSoil Science and Agrichemistry1999-740X2959-3433Казахский научно-исследовательский институт почвоведения и агрохимии имени У.У. Успановаkaz65-427Research ArticleSOIL BIOLOGY AND BIOCHEMISTRYEFFECT OF INOCULATES ON ABUNDANCE OF ACTINOMYCETES IN ALFALFA RHISOSPHERESnezanaAndjelkovicsnezana.andielkovic@ikbks.comTanjaVasicnoemail@neicon.ruJasminaRadovicnoemail@neicon.ruSnezanaBabicnoemail@neicon.ruZoranLugicnoemail@neicon.ruDraganTerzicnoemail@neicon.ruSimonidaDjuricnoemail@neicon.ruInstitute for Forage Crops,KazakhstanInstitute for Forage Crops, Krusevac, SerbiaKazakhstanUniversity of Novi Sad, Faculty of AgricultureKazakhstan201407112022044045Copyright © Snezana A., Tanja V., Jasmina R., Snezana B., Zoran L., Dragan T., Simonida D., 20222022Snezana A., Tanja V., Jasmina R., Snezana B., Zoran L., Dragan T., Simonida D.Snezana A., Tanja V., Jasmina R., Snezana B., Zoran L., Dragan T., Simonida D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.soil.kz/jour/article/view/427

В данной статье представлены результаты воздействия микробных биопрепаратов на число актиномицетов в ризосфере почвы под люцерну (Medicago SativaL.). Этот вид растений в дополнение к высокому потенциалу урожайности и качества биомассы, характеризуется интенсивным процессом фиксации азота. Ризосфера люцерны изобилует многочисленными микроорганизмами. Целью исследования было изучение влияния прививки двумя азотфиксирующими бактериями (Sinorhizobium meliloti и Azotobacter chroococcum) и двух изолятов (CC657 и Coll11) фитопатоген гриба Colletotrichum destructivum на количество актиномицетов в ризосферной почве под сортами люцерны (Аффинити, Перри и К-28).Наибольшее количество актиномицетов было определено в ризосфере сорта Аффинити, в которую вносили A. chroococcum + isolate Coll-11, в то время как наименьшее количество было обнаружено в ризосфере сорта К-28 привитым с CC657. Результаты теста Фишера показывает отсутствие статистически значимых различий в изобилии актиномицетов между обработками, что означает, что там было четыре однородные группы: 1. обработки сорта К-28 с Coll-11 с применением CC657 + S. meliloti; 2. обработки сорта К-28 при помощи Coll 11 + S. meliloti и контрольная обработка (без прививки) сорта Перри; 3. обработки сорта Перри с применением Coll 11 + A. chroococcum сорта К-28 и CC657 + A. chroococcum; 4. прививка К-28 с CC657 + A. chroococcum и сорта Перри при помощи Сб 11 + A. chroococcum.

This paper presents the results of the impact of microbial inoculants on the number of actinomycetes in the rhizosphere soil of alfalfa (Medicago sativa L.). This plant species in addition to high yield potential and quality of biomass, is characterized by an intense process of nitrogen fixation. The rhizosphere of alfalfa abounds in numerous microorganisms. The aim of the research was to investigate the effect of inoculating with two nitrogen-fixing bacteria (Sinorhizobium meliloti and Azotobacter chroococcum) and two isolates (CC657 and Coll11) ofthe phytopathogen fungus Colletotrichum destructivum on the number actinomycetes in the rhizospheric soil of alfalfa varieties (Affinity, Perry and K-28). The highest number of actinomycetes was determined in rhisosphere of cultivar Affinity which was inoculated with A. chroococcum + isolate Coll-11, while the lowest number was found in rhisosphere of cultivar K-28 inoculate with CC657. Results of Fisher test shows the absence of statistically significant differences in abundance of actinomycetes between treatments, meaning there were four homogenous groups: 1. treatments of cultivar K-28 with Coll-11 and CC657 + S. meliloti; 2. treatments of cultivar K-28 with Coll 11 + S. meliloti and control treatment (without inoculation) in cultivar Perry; 3. treatments with Coll 11 + A. chroococcum in cultivar K-28 and CC657 + A. chroococcum in cultivar Perry; 4. inoculation with CC657 + A. chroococcum in K-28 and Coll 11 + A. chroococcum in cultivar Perry.

RhizospherefungiactinomycetesalfalfaReferencesAndjelkovic, S., Jarak, M., Radovic, J., Vasic, T., Milenkovic, J., Babic, S., 2010. The number of azotobacter in the rhizosphere soil of different alfalfa genotypes.Contemporary agriclture 59 (1-2), 126-131.Andjelkovic, S., Jarak, M., Radovic, J., Vasic, T., Milenkovic, J., Babic, S., 2010. The number of azotobacter in the rhizosphere soil of different alfalfa genotypes.Contemporary agriclture 59 (1-2), 126-131.Aldesuquy, H.S., Mansour, F.A., Abo-Hamed, S.A., 1998. Effect of the culture filtrates of Streptomyces on growth and productivity ofwheat plants. Folia Microbiologica, 43, 465-470.Aldesuquy, H.S., Mansour, F.A., Abo-Hamed, S.A., 1998. Effect of the culture filtrates of Streptomyces on growth and productivity ofwheat plants. Folia Microbiologica, 43, 465-470.Avis, J.T., Gravel, V., Antoun, H., Russell J., Tweddell, J.R., 2008. Multifaceted beneficial effects of rhizosphere microorganisms on plant health and productivity. Soil Biology & Biochemistry 40, 1733-1740.Avis, J.T., Gravel, V., Antoun, H., Russell J., Tweddell, J.R., 2008. Multifaceted beneficial effects of rhizosphere microorganisms on plant health and productivity. Soil Biology & Biochemistry 40, 1733-1740.Andjelkovic S., Vasic T., Radovic J., Babic S. Jarak M., Djuric S., Ciric S., 2013. Effect of inoculates on abudanceog fungi and actinomycetes in alfalfa rhizosphere. Proceedings of The 1st International Congress on Soil Science, 23-26 September, Belgrade, Republic of Serbia, 232238.Andjelkovic S., Vasic T., Radovic J., Babic S. Jarak M., Djuric S., Ciric S., 2013. Effect of inoculates on abudanceog fungi and actinomycetes in alfalfa rhizosphere. Proceedings of The 1st International Congress on Soil Science, 23-26 September, Belgrade, Republic of Serbia, 232238.Boland, G.J., Brochu, L.D., 1989. Colletotrichum destructivum on alfalfa in Ontario and cultivar response to anthracnose. Canadian Journal Plant Pathol 11, 303-307.Boland, G.J., Brochu, L.D., 1989. Colletotrichum destructivum on alfalfa in Ontario and cultivar response to anthracnose. Canadian Journal Plant Pathol 11, 303-307.Bredholt, H., Fjaervik, E., Jhonsen, G., Zotechev, S.B. 2008. Actinomycetes from sediments in the Trondhein Fjrod, Norway: Diversity and biological activity. Journal of Marine Drugs 6, 1224.Bredholt, H., Fjaervik, E., Jhonsen, G., Zotechev, S.B. 2008. Actinomycetes from sediments in the Trondhein Fjrod, Norway: Diversity and biological activity. Journal of Marine Drugs 6, 1224.Denison, R,F, Kiers, E,T, 2004. Why are most rhizobia beneficial to their host plants, rather than parasitic, Microbes and Infection 6, 1235-1239.Denison, R,F, Kiers, E,T, 2004. Why are most rhizobia beneficial to their host plants, rather than parasitic, Microbes and Infection 6, 1235-1239.Dobbelaere, S., Vanderleyden, J., Okon, Y., 2003. Plant growth-promoting effects of diazotrophs in the rhizosphere. Crit. Rev. Plant Sci. 22, 107-149.Dobbelaere, S., Vanderleyden, J., Okon, Y., 2003. Plant growth-promoting effects of diazotrophs in the rhizosphere. Crit. Rev. Plant Sci. 22, 107-149.Egamberdiyeva, D., 2007. The effect of plant growth promoting bacteria on growth an nutrient uptake ofmaize in two different soils, applied soil ecology, 36,184-189.Egamberdiyeva, D., 2007. The effect of plant growth promoting bacteria on growth an nutrient uptake ofmaize in two different soils, applied soil ecology, 36,184-189.Fermino - Soares, A.C., da Silva Sousa, C, da Silva Garrido, M Oliveira Perez, J., 2007. Production of streptomycete inoculum in sterilized rice. Sci. Agric. (Piracicaba, Braz.), Vol. 64 (6), 641-644.Fermino - Soares, A.C., da Silva Sousa, C, da Silva Garrido, M Oliveira Perez, J., 2007. Production of streptomycete inoculum in sterilized rice. Sci. Agric. (Piracicaba, Braz.), Vol. 64 (6), 641-644.Getha, K., Vikineswary, S., Wong, W.H., Seki, T., Ward, A., Goodfellow, M., 2005.Evaluation of Streptomyces sp. Straing 10 for suppression ofFusarium wilt and rhizosphere colonization in pot grown banana plantlets. Journal of Indian Microbiology and Biotechnology, Vol.32, 24-32.Getha, K., Vikineswary, S., Wong, W.H., Seki, T., Ward, A., Goodfellow, M., 2005.Evaluation of Streptomyces sp. Straing 10 for suppression ofFusarium wilt and rhizosphere colonization in pot grown banana plantlets. Journal of Indian Microbiology and Biotechnology, Vol.32, 24-32.Gharib, A.A., Shahen, M.M., Ragab, A.A., 2009. Influence of Rhizobium Inoculation combined with Azotobacter chroococcum and Bacillus megaterium var phosphaticum on growth, nodulation, yield and quality of two snap been (Phasealus vulgaris l.) cultivars. Proceedings of the 4th Conference on Recent Technologies in Agriculture, 3-5 November 2009, Cairo, Egypt. Vol. 4, 650-661.Gharib, A.A., Shahen, M.M., Ragab, A.A., 2009. Influence of Rhizobium Inoculation combined with Azotobacter chroococcum and Bacillus megaterium var phosphaticum on growth, nodulation, yield and quality of two snap been (Phasealus vulgaris l.) cultivars. Proceedings of the 4th Conference on Recent Technologies in Agriculture, 3-5 November 2009, Cairo, Egypt. Vol. 4, 650-661.Janvier, C., Villeneuve, F., Alabouvette, C., Edel-Hermann, V., Mateille, T., Steinberg, C., 2007. Soil health through soil disease suppression: which strategy from descriptors to indicators. Soil Biol. Biochem. 39, 1-23.Janvier, C., Villeneuve, F., Alabouvette, C., Edel-Hermann, V., Mateille, T., Steinberg, C., 2007. Soil health through soil disease suppression: which strategy from descriptors to indicators. Soil Biol. Biochem. 39, 1-23.Jarak, M., Mrkovacki, N., Bjelic, D., Josic, D., Hajnal-Jafari, T., Stamenov, D., 2012. Effects of Plant growth promoting rhizobacteria on maize in greenhouse and field trial. African Journal Of Microbiology Research 6(27), 5683-5690.Jarak, M., Mrkovacki, N., Bjelic, D., Josic, D., Hajnal-Jafari, T., Stamenov, D., 2012. Effects of Plant growth promoting rhizobacteria on maize in greenhouse and field trial. African Journal Of Microbiology Research 6(27), 5683-5690.Jeon, J.S., Lee, S.S., Kim, H.Y., Ahn, T.S,. Song, H.G., 2003. Plant growth promotion in soil by some inoculated microorganisms. Journal of Microbiology, 41(4), 271-276.Jeon, J.S., Lee, S.S., Kim, H.Y., Ahn, T.S,. Song, H.G., 2003. Plant growth promotion in soil by some inoculated microorganisms. Journal of Microbiology, 41(4), 271-276.Jarak, M., Djuric, S., 2006. Praktikum iz mikrobiologije. Univerzitet u Novom Sadu, Poljoprivredni fakultet.Jarak, M., Djuric, S., 2006. Praktikum iz mikrobiologije. Univerzitet u Novom Sadu, Poljoprivredni fakultet.Macek, T., Mackova, M., Kas, J., 2000. Research review paper exploitation of plants for the removal of organics in environmental remediation. Biotechnology Advances 18, 23-34.Macek, T., Mackova, M., Kas, J., 2000. Research review paper exploitation of plants for the removal of organics in environmental remediation. Biotechnology Advances 18, 23-34.Minuto, A.,Spadaro, D., Garibaldi, A., Gullino, M.L., 2006. Control of soilborne pathogen of tomato using a commercial formulation of Streptomyces griseoviridis and solarization Crop Protection 25, 468-475.Minuto, A.,Spadaro, D., Garibaldi, A., Gullino, M.L., 2006. Control of soilborne pathogen of tomato using a commercial formulation of Streptomyces griseoviridis and solarization Crop Protection 25, 468-475.Nolan, R.D., Cross, T., 1988. Isolation and screening of actinomycetes. Actinomycetes in biotechnology. Orlando: Academic Press.Nolan, R.D., Cross, T., 1988. Isolation and screening of actinomycetes. Actinomycetes in biotechnology. Orlando: Academic Press.Oskay, M., Tamer, A.U., Azeri, C., 2004. Antibacterial activity of some Actinomycetes isolated from farming soils of Turkey. African Journal of Biotechnology, 3, 9 441- 446.Oskay, M., Tamer, A.U., Azeri, C., 2004. Antibacterial activity of some Actinomycetes isolated from farming soils of Turkey. African Journal of Biotechnology, 3, 9 441- 446.Peoples, M.B., Herridge, D.F., Ladha, J.K., 1995. Biological nitrogen fixation: an efficient source ofnitrogen for sustainable agricultural production. Plant and Soil 174, 3-28.Peoples, M.B., Herridge, D.F., Ladha, J.K., 1995. Biological nitrogen fixation: an efficient source ofnitrogen for sustainable agricultural production. Plant and Soil 174, 3-28.Raaijmakers, J., Paulitz, T., Steinberg, C., Alabouvette, C, Moёnne-Loccoz, Y., 2009. The rhizosphere: a playground and battlefield for soilborne pathogens and beneficial microorganisms. Plant Soil 321, 341-361.Raaijmakers, J., Paulitz, T., Steinberg, C., Alabouvette, C, Moёnne-Loccoz, Y., 2009. The rhizosphere: a playground and battlefield for soilborne pathogens and beneficial microorganisms. Plant Soil 321, 341-361.Vasic, T., Radovic, J., Lugic, Z., Markovic, J., Jevtic, G., Gajic, S.,2009. Occurrence of Colletotrichum trifolii (Bain et Essary), the inducer of alfalfa anthracnose in Serbia. In: C Huyghe (ed.) Sustainable Use of Genetic Diversity in Forage and Turf Breeding. Springer. 53, 369-374.Vasic, T., Radovic, J., Lugic, Z., Markovic, J., Jevtic, G., Gajic, S.,2009. Occurrence of Colletotrichum trifolii (Bain et Essary), the inducer of alfalfa anthracnose in Serbia. In: C Huyghe (ed.) Sustainable Use of Genetic Diversity in Forage and Turf Breeding. Springer. 53, 369-374.Williams, J.R., Jones, C.A., Dyke, P.T., 1984. A modeling approach to determining the relationship between erosion and soil productivity. Trans. Asae 27(1), 129-144.Williams, J.R., Jones, C.A., Dyke, P.T., 1984. A modeling approach to determining the relationship between erosion and soil productivity. Trans. Asae 27(1), 129-144.

The authors declare that there are no conflicts of interest present.